Macintosh Genophiler Spreadsheet Summary

Sample Name GT Results D3 vWA FGA D16
Victim
Vic PP		 Blue GenoTyper Lab 16 (151)
17 (132)
14 (254)
19 (256)
21 (166)
24 (260)
_
Victim-C
Vic CO		 Blue GenoTyper Lab 16 (227)
17 (267)
_ _ 9 (99)
13 (136)
Defendant
Def P		 Blue GenoTyper Lab 15 (714)
17 (540)
13 (729)
19 (682)
22 (629)
24 (501)
_
Defendant-C
Def C		 Blue GenoTyper Lab 15 (533)
17 (399)
_ _ 11 (503)
13 (526)
Cigarette
Cigarette P		 Blue GenoTyper Lab 15 (2018)
17 (1932)
13 (2064)
19 (1681)
22 (879)
24 (757)
_
Cigarette-C
Cigarette C		 Blue GenoTyper Lab 15 (2330)
17 (2109)
_ _ 11 (1462)
13 (1405)
Knife Blade
Knife Blade		 Blue GenoTyper Lab 15 (4950)
16 (51)
17 (160)
18 (71)
14 (106)
15 (195)
16 (1070)
17 (182)
18 (148)
19 (933)
19 (117)
20 (123)
21 (99)
23 (615)
24 (484)
25 (63)
_
Knife Handle
Knife Handle		 Blue GenoTyper Lab 15 (2062)
16 (80)
14 (336)
15 (63)
16 (307)
17 (351)
19 (188)
20 (114)
21 (360)
23 (328)
24 (529)
_
Underwear
Underwear PP		 Blue GenoTyper Lab 11 (69)
15 (968)
16 (1056)
17 (1053)
18 (177)
14 (854)
15 (90)
19 (915)
19 (259)
21 (605)
22 (244)
24 (480)
_
Underwear-C
Underwear CO		 Blue GenoTyper Lab 15 (423)
16 (625)
17 (517)
18 (64)
_ _ 9 (378)
10 (143)
11 (156)
13 (363)
Known Blank (Run 4)
KB		 Blue GenoTyper Lab _ _ 21 (76)
_
Neg Control (Run 1)
NEG - PP		 Blue GenoTyper Lab _ _ _ _
Neg Control (Run 4)
Neg		 Blue GenoTyper Lab _ _ 20.2 (50)
21 (71)
_
Pos Control (Run 1)
POS CTRL - PP		 Blue GenoTyper Lab 14 (2117)
15 (1827)
17 (1795)
18 (1827)
23 (1584)
24 (1215)
_
Pos Control (Run 1)-C
POS CTRL - CO		 Blue GenoTyper Lab 14 (1993)
15 (1939)
_ _ 11 (1385)
12 (1451)
Pos Control (Run 2)-C
POS CTRL - CO		 Blue GenoTyper Lab 14 (804)
15 (772)
_ _ 11 (944)
12 (759)
Pos Control (Run 3)
POS CTL P		 Blue GenoTyper Lab 14 (1299)
15 (1187)
17 (986)
18 (799)
23 (983)
24 (852)
_
Pos Control (Run 3)-C
POS CTL C		 Blue GenoTyper Lab 14 (1322)
15 (1282)
_ _ 11 (1361)
12 (1123)
Pos Control (Run 4)
Pos Control		 Blue GenoTyper Lab 17 (1937)
19 (1613)
15 (1602)
16 (1468)
21 (76)
23 (1468)
28 (1225)
_
Pos Control (Run2)
POS CTRL - PP		 Blue GenoTyper Lab 11 (98)
14 (2772)
15 (2635)
17 (2216)
18 (2257)
23 (1889)
24 (1767)
_
Question Blank (Run 4)
QB		 Blue GenoTyper Lab _ 14 (129)
21 (76)
_

 

Sample Name GT Results Amel D8 D21 D18 THO1 TPOX CSF
Victim
Vic PP		 Green GenoTyper Lab X (223)
Y (362)
14 (258)
30 (297)
14 (104)
16 (81)
_ _ _
Victim-C
Vic CO		 Green GenoTyper Lab X (540)
Y (606)
_ _ _ 8 (52)
6 (54)
8 (101)
11 (50)
12 (53)
Defendant
Def P		 Green GenoTyper Lab X (1212)
Y (963)
15 (1738)
28 (839)
29 (756)
15 (475)
20 (519)
_ _ _
Defendant-C
Def C		 Green GenoTyper Lab X (762)
Y (679)
_ _ _ 9 (626)
9 (415)
11 (343)
8 (342)
10 (304)
Cigarette
Cigarette P		 Green GenoTyper Lab X (4727)
Y (4327)
15 (6229)
28 (1801)
29 (1805)
15 (693)
20 (425)
_ _ _
Cigarette-C
Cigarette C		 Green GenoTyper Lab X (3051)
Y (2952)
_ _ _ 9 (3968)
9 (1870)
11 (1940)
8 (1667)
10 (1376)
Knife Blade
Knife Blade		 Green GenoTyper Lab X (5307)
Y (4460)
8 (120)
10 (215)
13 (2589)
14 (2336)
15 (187)
29 (66)
30 (261)
30.2 (899)
31 (716)
32 (93)
32.2 (60)
12 (79)
13 (65)
14 (366)
16 (283)
_ _ _
Knife Handle
Knife Handle		 Green GenoTyper Lab X (1765)
Y (1693)
12 (177)
13 (1171)
14 (881)
15 (52)
28 (69)
29 (67)
30 (396)
30.2 (752)
31 (306)
13 (197)
14 (188)
15 (233)
16 (164)
_ _ _
Underwear
Underwear PP		 Green GenoTyper Lab X (1536)
Y (1709)
9 (88)
10 (294)
14 (1667)
27 (288)
29 (249)
30 (1226)
32.2 (53)
14 (339)
16 (749)
17 (226)
_ _ _
Underwear-C
Underwear CO		 Green GenoTyper Lab X (967)
Y (814)
_ _ _ 6 (125)
8 (455)
9 (426)
9.3 (182)
8 (934)
9 (137)
10 (144)
11 (543)
12 (282)
Known Blank (Run 4)
KB		 Green GenoTyper Lab _ _ _ _ _ _ _
Neg Control (Run 1)
NEG - PP		 Green GenoTyper Lab _ 19 (100)
_ _ _ _ _
Neg Control (Run 4)
Neg		 Green GenoTyper Lab _ _ 38.2 (50)
_ _ _ _
Pos Control (Run 1)
POS CTRL - PP		 Green GenoTyper Lab X (3262)
13 (2830)
30 (2548)
15 (1185)
19 (1165)
_ _ _
Pos Control (Run 1)-C
POS CTRL - CO		 Green GenoTyper Lab X (3926)
_ _ _ 8 (997)
9.3 (870)
8 (2240)
10 (1207)
12 (1161)
Pos Control (Run 2)-C
POS CTRL - CO		 Green GenoTyper Lab X (1725)
_ _ _ 8 (263)
9.3 (270)
8 (1418)
10 (620)
12 (631)
Pos Control (Run 3)
POS CTL P		 Green GenoTyper Lab X (3328)
13 (2616)
30 (1767)
15 (1110)
19 (970)
_ _ _
Pos Control (Run 3)-C
POS CTL C		 Green GenoTyper Lab X (2725)
_ _ _ 8 (1151)
9.3 (1054)
8 (2508)
10 (1303)
12 (1380)
Pos Control (Run 4)
Pos Control		 Green GenoTyper Lab X (2136)
Y (2102)
13 (4074)
29 (2096)
30 (2045)
13 (1743)
14 (1632)
_ _ _
Pos Control (Run2)
POS CTRL - PP		 Green GenoTyper Lab X (6177)
13 (4770)
30 (3507)
15 (1869)
19 (1490)
_ _ _
Question Blank (Run 4)
QB		 Green GenoTyper Lab _ _ _ _ _ _ _

 

Sample Name GT Results D5 D13 D7
Victim
Vic PP		 Yellow GenoTyper Lab 11 (376)
12 (288)
11 (98)
12 (86)
10 (50)
11 (51)
Victim-C
Vic CO		 Yellow GenoTyper Lab _ _ 10 (154)
11 (147)
Defendant
Def P		 Yellow GenoTyper Lab 12 (2572)
12 (791)
13 (670)
8 (583)
11 (605)
Defendant-C
Def C		 Yellow GenoTyper Lab _ _ 8 (375)
11 (277)
Cigarette
Cigarette P		 Yellow GenoTyper Lab 12 (5850)
12 (2209)
13 (1981)
8 (541)
11 (374)
Cigarette-C
Cigarette C		 Yellow GenoTyper Lab _ _ 8 (1225)
11 (1125)
Knife Blade
Knife Blade		 Yellow GenoTyper Lab 10 (2048)
11 (246)
12 (471)
13 (1748)
8 (708)
9 (91)
11 (171)
12 (599)
10 (92)
11 (269)
12 (234)
Knife Handle
Knife Handle		 Yellow GenoTyper Lab 10 (397)
11 (549)
12 (186)
13 (944)
8 (163)
9 (289)
11 (432)
12 (306)
10 (238)
11 (141)
12 (327)
Underwear
Underwear PP		 Yellow GenoTyper Lab 10 (319)
11 (1002)
12 (1288)
8 (249)
11 (654)
12 (677)
10 (749)
11 (443)
12 (149)
Underwear-C
Underwear CO		 Yellow GenoTyper Lab _ _ 9 (58)
10 (472)
11 (251)
12 (129)
Known Blank (Run 4)
KB		 Yellow GenoTyper Lab _ _ _
Neg Control (Run 1)
NEG - PP		 Yellow GenoTyper Lab _ _ _
Neg Control (Run 4)
Neg		 Yellow GenoTyper Lab _ _ _
Pos Control (Run 1)
POS CTRL - PP		 Yellow GenoTyper Lab 11 (2922)
11 (2323)
10 (622)
11 (870)
Pos Control (Run 1)-C
POS CTRL - CO		 Yellow GenoTyper Lab _ _ 10 (833)
11 (762)
Pos Control (Run 2)-C
POS CTRL - CO		 Yellow GenoTyper Lab _ _ 10 (428)
11 (376)
Pos Control (Run 3)
POS CTL P		 Yellow GenoTyper Lab 11 (2925)
12 (52)
11 (2471)
10 (843)
11 (809)
Pos Control (Run 3)-C
POS CTL C		 Yellow GenoTyper Lab _ _ 10 (1132)
11 (985)
Pos Control (Run 4)
Pos Control		 Yellow GenoTyper Lab 9 (1835)
12 (1862)
8 (1655)
11 (1754)
9 (1379)
13 (1178)
Pos Control (Run2)
POS CTRL - PP		 Yellow GenoTyper Lab 11 (4877)
11 (2873)
10 (1314)
11 (1250)
Question Blank (Run 4)
QB		 Yellow GenoTyper Lab _ _ _

 

Samples Not Labeled by GenoTyper
Sample Run Sample Name Sample Run Sample Name
Run1NEG - CO Run2NEG - CO
Run3NEG CTL P Run3RBC P
Run3NEG CTL C Run3RBC C
Run4GS500 __

 

Sample Name Sample Nickname Low PH (<150) High PH (>4000) X/Y Ratio Indication of Mixture Possible Pull-Up PH Imbalance
Vic PP Victim
7 _ 62% _ _ FGA (64%)
Vic CO Victim-C
8 _ 89% _ _ TPOX (53%)
Def P Defendant
_ _ 126% _ _ _
Def C Defendant-C
_ _ 112% _ _ _
Cigarette P Cigarette
_ 2 109% _ _ D18 (61%) , D7 (69%)
Cigarette C Cigarette-C
_ _ 103% _ _ _
Knife Blade Knife Blade
16 1 119% D3 , vWA , FGA , D8 , D21 , D18 , D5 , D13 , D7 _ _
Knife Handle Knife Handle
7 _ 104% vWA , FGA , D8 , D21 , D18 , D5 , D13 , D7 _ _
Underwear PP Underwear
5 _ 90% D3 , vWA , FGA , D8 , D21 , D18 , D5 , D13 , D7 _ _
Underwear CO Underwear-C
7 _ 119% D3 , D16 , THO1 , CSF , D7 _ TPOX (15%)
KB Known Blank (Run 4)
1 _ NA _ _ _
NEG - PP Neg Control (Run 1)
1 _ NA _ _ _
Neg Neg Control (Run 4)
3 _ NA _ _ _
POS CTRL - PP Pos Control (Run 1)
_ _ X Only _ _ _
POS CTRL - CO Pos Control (Run 1)-C
_ _ X Only _ _ _
POS CTRL - CO Pos Control (Run 2)-C
_ _ X Only _ _ _
POS CTL P Pos Control (Run 3)
1 _ X Only _ _ _
POS CTL C Pos Control (Run 3)-C
_ _ X Only _ _ _
Pos Control Pos Control (Run 4)
1 1 102% FGA _ _
POS CTRL - PP Pos Control (Run2)
1 2 X Only D3 _ _
QB Question Blank (Run 4)
2 _ NA _ _ _

 

Sample Name Sample Nickname Degradation Average PH PP/CO Discrepancy
Vic PP Victim _ 182 _
Vic CO Victim-C _ 122 _
Def P Defendant Y (-12.10) 834 _
Def C Defendant-C _ 422 _
Cigarette P Cigarette B (-21.89),
G (-32.13),
Y (-40.65)		 1,952 _
Cigarette C Cigarette-C B (-12.11),
G (-8.69)		 1,862 _
Knife Blade Knife Blade _ 580 _
Knife Handle Knife Handle _ 381 _
Underwear PP Underwear _ 564 Yes
Underwear CO Underwear-C _ 324 Yes
KB Known Blank (Run 4) _ 76 _
NEG - PP Neg Control (Run 1) _ 100 _
Neg Neg Control (Run 4) _ 57 _
POS CTRL - PP Pos Control (Run 1) B (-9.73),
Y (-11.27)		 1,774 _
POS CTRL - CO Pos Control (Run 1)-C B (-8.20) 1,349 _
POS CTRL - CO Pos Control (Run 2)-C _ 662 _
POS CTL P Pos Control (Run 3) Y (-10.01) 1,311 _
POS CTL C Pos Control (Run 3)-C _ 1,327 _
Pos Control Pos Control (Run 4) _ 1,702 _
POS CTRL - PP Pos Control (Run2) _ 2,372 _
QB Question Blank (Run 4) _ 103 _

 

 Allele Coloring Key:
   - Red: Allele Shared with Victim
   - Blue: Allele Shared with Defendant
   - Green: Allele Shared with Victim and Defendant
   - Black: Allele Not Shared with Victim or Defendant

 Notes:
   - Sample nicknames ending with '-C' contain the results of the COfiler analysis
   - The Low, High, and Average PH (peak height) calculations only include on-ladder alleles.
   - The Low PH count corresponds to the number of alleles called in a sample between 50 and 150 RFUs.
   - The High PH count corresponds to the number of alleles called in a sample greater than 4,000 RFUs.
   - Possible mixtures note those loci within a sample where more than two on-ladder alleles are observed.
   - Allele calls that may be due to pull-up are those for which the size measurement of two peaks within
      a sample are observed to be within 0.04 base pairs.
   - Potentially significant PH Imbalance is noted for those samples in which there are only two on-ladder alleles
      at a locus and the ratio of their peak heights is less than 0.70.
   - Degradation is measured using linear regression of on-ladder alleles. Samples are flagged if their slope is less than -8 and
      do not contain a mixture.
   - Asterisks are indicative of possible pull-up. Use the letters as a guide to determine which peaks are possibly being caused by other larger peaks in other dyes.

 

Questions? Contact Dan Krane, Krane@bioforensics.com.

Creation Date: 2/11/2005 3:32 pm