Genophiler Spreadsheet Summary

Sample Name GT Results D3 vWA FGA D16
BLANK run1 Blue GenoTyper Lab _ _ _ _
POSITIVE PP run1 Blue GenoTyper Lab 14 (1399)   15 (1282)   16 (104)   OL (53)   17 (1358)   18 (1084)   23 (1143)   24 (911)   _
NEGATIVE PP run1 Blue GenoTyper Lab _ _ _ _
Evidence2 PP Blue GenoTyper Lab 14 (1161)   15 (1084)   15 (64)   OL (64)   16 (1052)   18 (82)   OL (50)   19 (832)   21 (1207)   _
Evidence1 PP Blue GenoTyper Lab 14 (851)   15 (759)   16 (721)   19 (660)   21 (1150)   _
Defendant PP Blue GenoTyper Lab 13 (159)   OL (62)   14*a (2820)   19 (58)   14 (75)   OL (54)   15 (1234)   OL (72)   16 (1301)   22 (881)   24 (765)   OL (52)   _
Victim PP Blue GenoTyper Lab 14 (1079)   15 (926)   16*bc (102)   16 (755)   19 (664)   21 (1284)   _
POSITIVE CO run1 Blue GenoTyper Lab 14 (1032)   15 (858)   _ _ 11 (1076)   12 (889)  
Evidence3 CO Blue GenoTyper Lab 14 (669)   15 (640)   _ _ 13 (774)  
Evidence1 CO Blue GenoTyper Lab 14 (422)   15 (380)   _ _ 13 (716)  
Defendant CO Blue GenoTyper Lab OL (51)   14 (2168)   _ _ 9 (805)   12 (675)  
Victim CO Blue GenoTyper Lab 14 (858)   15 (794)   _ _ 13 (1034)  
blank run2 Blue GenoTyper Lab _ _ _ _
neg_ cont_ co run2 Blue GenoTyper Lab _ _ _ _
pos_ cont_ co run2 Blue GenoTyper Lab 14 (989)   15 (871)   OL (50)   _ _ 11 (804)   12 (855)  
Evidence2 CO Blue GenoTyper Lab 14 (1003)   15 (895)   _ _ 13 (1219)  
blank run3 Blue GenoTyper Lab _ OL (101)   _ _
pos_ cont_ pp run3 Blue GenoTyper Lab 14 (1262)   15 (1129)   16 (94)   OL (62)   17 (1253)   OL (69)   18 (1243)   23 (901)   24 (890)   _
Evidence3 PP Blue GenoTyper Lab 14 (1032)   15 (1089)   16 (822)   19 (690)   21 (1071)   _

 

Sample Name GT Results Amel D8 D21 D18 THO1 TPOX CSF
BLANK run1 Green GenoTyper Lab _ _ _ 18 (53)   OL (55)   _ _ _
POSITIVE PP run1 Green GenoTyper Lab OL (112)   X (3207)   8 (51)   12 (182)   OL (84)   13 (2689)   OL (61)   29 (138)   OL (61)   30 (1949)   31 (60)   38 (53)   15 (1303)   19 (946)   _ _ _
NEGATIVE PP run1 Green GenoTyper Lab _ OL (52)   _ _ _ _ _
Evidence2 PP Green GenoTyper Lab X (3021)   13 (65)   OL (53)   14 (1031)   15 (102)   OL (56)   16 (1025)   27 (877)   32.2 (674)   12 (68)   16 (1051)   _ _ _
Evidence1 PP Green GenoTyper Lab X (2173)   OL (59)   11 (50)   OL (56)   14 (792)   16 (828)   27 (663)   32.2 (671)   16 (1292)   OL (57)   _ _ _
Defendant PP Green GenoTyper Lab X (1898)   OL (67)   Y (1668)   7*a (52)   11 (105)   OL (108)   12 (3025)   28 (1129)   29 (996)   17 (1463)   OL (52)   OL (56)   OL (53)   19 (53)   _ _ _
Victim PP Green GenoTyper Lab X (2391)   OL*bd (55)   OL (54)   OL (67)   14 (859)   16 (852)   27 (806)   32.2 (695)   34.2 (56)   16 (917)   OL (65)   OL (56)   _ _ _
POSITIVE CO run1 Green GenoTyper Lab X (2212)   _ _ _ 8 (734)   9.3 (659)   7 (52)   OL (75)   8 (1986)   OL (62)   10 (841)   12 (872)  
Evidence3 CO Green GenoTyper Lab OL (75)   X (1923)   _ _ _ 7 (451)   8 (402)   9 (596)   10 (521)   11 (644)  
Evidence1 CO Green GenoTyper Lab OL (53)   X (1116)   _ _ _ 7 (329)   8 (284)   9 (427)   10 (448)   11 (544)  
Defendant CO Green GenoTyper Lab OL (58)   X (1348)   OL (75)   Y (1313)   _ _ _ OL (55)   OL (56)   9.3 (1393)   8 (1004)   9 (936)   10 (614)   11 (60)   12 (557)  
Victim CO Green GenoTyper Lab OL (92)   X (2110)   OL (73)   _ _ _ 7 (553)   8 (478)   9 (715)   10 (769)   11 (889)   13 (51)   OL (53)  
blank run2 Green GenoTyper Lab OL (57)   _ _ _ OL (69)   OL (56)   OL (55)   11 (56)   OL (56)   _ OL (50)   OL (56)  
neg_ cont_ co run2 Green GenoTyper Lab _ _ _ _ OL (56)   _ OL (56)  
pos_ cont_ co run2 Green GenoTyper Lab OL (81)   X (1868)   _ _ _ 8 (721)   9.3 (798)   8 (1589)   OL (51)   10 (652)   12 (641)  
Evidence2 CO Green GenoTyper Lab OL (84)   X (2793)   _ _ _ 7 (617)   8 (565)   9 (55)   9 (740)   10 (700)   11 (881)  
blank run3 Green GenoTyper Lab OL (64)   OL (51)   25 (50)   OL (53)   _ _ _
pos_ cont_ pp run3 Green GenoTyper Lab OL (138)   X (3392)   8 (60)   13 (2772)   OL (55)   29 (134)   OL (61)   30 (2026)   15 (1104)   19 (1069)   OL (51)   _ _ _
Evidence3 PP Green GenoTyper Lab OL (93)   X (2376)   14 (1058)   16 (834)   27 (660)   32.2 (586)   OL (55)   OL (51)   OL (54)   16 (964)   _ _ _

 

Sample Name GT Results D5 D13 D7
BLANK run1 Yellow GenoTyper Lab _ _ _
POSITIVE PP run1 Yellow GenoTyper Lab 11 (2411)   11 (1486)   10 (618)   11 (684)  
NEGATIVE PP run1 Yellow GenoTyper Lab _ _ _
Evidence2 PP Yellow GenoTyper Lab 11 (1823)   11 (661)   12 (584)   8 (441)   10 (424)  
Evidence1 PP Yellow GenoTyper Lab 11 (1521)   11 (531)   12 (483)   8 (452)   10 (460)  
Defendant PP Yellow GenoTyper Lab 11 (1381)   12 (1343)   11 (910)   14 (803)   10 (1213)  
Victim PP Yellow GenoTyper Lab 6*cd (57)   11 (1778)   11 (590)   12 (603)   OL (55)   8 (473)   10 (374)  
POSITIVE CO run1 Yellow GenoTyper Lab _ _ 10 (542)   11 (482)  
Evidence3 CO Yellow GenoTyper Lab _ _ 8 (271)   10 (247)  
Evidence1 CO Yellow GenoTyper Lab _ _ 8 (270)   10 (286)  
Defendant CO Yellow GenoTyper Lab _ _ 10 (987)  
Victim CO Yellow GenoTyper Lab _ _ 8 (407)   10 (373)  
blank run2 Yellow GenoTyper Lab _ _ _
neg_ cont_ co run2 Yellow GenoTyper Lab _ _ _
pos_ cont_ co run2 Yellow GenoTyper Lab _ _ 10 (540)   11 (457)  
Evidence2 CO Yellow GenoTyper Lab _ _ 8 (481)   10 (402)  
blank run3 Yellow GenoTyper Lab _ _ _
pos_ cont_ pp run3 Yellow GenoTyper Lab 11 (2618)   11 (1639)   10 (767)   11 (675)  
Evidence3 PP Yellow GenoTyper Lab 11 (1762)   11 (531)   12 (548)   8 (429)   10 (400)  

 

Sample Name Sample Nickname Low PH (<150) High PH (>4000) X/Y Ratio Indication of Mixture Possible Pull-Up PH Imbalance
BLANK run1 _ 1 _ NA _ _ _
POSITIVE PP run1 _ 5 _ X Only vWA , D8 , D21 _ _
NEGATIVE PP run1 _ _ _ NA _ _ _
Evidence2 PP _ 5 _ X Only vWA , D8 _ D18
Evidence1 PP _ 1 _ X Only D8 _ _
Defendant PP _ 5 _ 114% D3 , vWA , D8 Yes D18
Victim PP _ 3 _ X Only D3 , D21 Yes D5
POSITIVE CO run1 _ 1 _ X Only _ _ TPOX
Evidence3 CO _ _ _ X Only _ _ _
Evidence1 CO _ _ _ X Only _ _ _
Defendant CO _ 1 _ 103% CSF _ _
Victim CO _ 1 _ X Only _ _ CSF
blank run2 _ 1 _ NA _ _ _
neg_ cont_ co run2 _ _ _ NA _ _ _
pos_ cont_ co run2 _ _ _ X Only _ _ _
Evidence2 CO _ 1 _ X Only THO1 _ _
blank run3 _ 1 _ NA _ _ _
pos_ cont_ pp run3 _ 3 _ X Only vWA _ D8 , D21
Evidence3 PP _ _ _ X Only _ _ _

 

Sample Name Sample Nickname Degradation Average PH PP/CO Discrepancy
BLANK run1 _ _ 53 _
POSITIVE PP run1 _ _ 1,098 _
NEGATIVE PP run1 _ _ _ _
Evidence2 PP _ _ 825 _
Evidence1 PP _ _ 827 _
Defendant PP _ _ 1,061 _
Victim PP _ _ 803 _
POSITIVE CO run1 _ _ 941 _
Evidence3 CO _ G (-5.75) 649 _
Evidence1 CO _ _ 475 _
Defendant CO _ _ 988 _
Victim CO _ G (-5.23) 753 _
blank run2 _ G (-6.28) 56 _
neg_ cont_ co run2 _ _ _ _
pos_ cont_ co run2 _ _ 899 _
Evidence2 CO _ _ 863 _
blank run3 _ _ 50 _
pos_ cont_ pp run3 _ _ 1,279 _
Evidence3 PP _ B (-9.84),
G (-6.58),
Y (-7.93)
928 _

 

 Allele Coloring Key:
   - Red: Allele Shared with Victim
   - Blue: Allele Shared with Defendant
   - Green: Allele Shared with Victim and Defendant
   - Black: Allele Not Shared with Victim or Defendant

 Notes:
   - The Low, High, and Average PH (peak height) calculations only include on-ladder alleles.
   - The Low PH count corresponds to the number of alleles called in a sample between 50 and 150 RFUs.
   - The High PH count corresponds to the number of alleles called in a sample greater than 4,000 RFUs.
   - Possible mixtures note those loci within a sample where more than two on-ladder alleles are observed.
   - Allele calls that may be due to pull-up are those for which the size measurement of two peaks within
      a sample are observed to be within 0.04 base pairs.
   - Potentially significant PH Imbalance is noted for those samples in which there are only two on-ladder alleles
      at a locus and the ratio of their peak heights is less than 0.70.
   - Degradation is measured using linear regression of on-ladder alleles. Samples are flagged if their slope is less than -5 and
      do not contain a mixture. Noise can account for flagged samples, so it is advisable to review the GenoTyper electropherograms.
   - Asterisks are indicitive of possible pull-up. Use the letters as a guide to determine which peaks are possibly being caused by other larger peaks in other dyes.

 

Questions? Contact Dan Krane, Krane@bioforensics.com.

Creation Date: 1/10/2003 2:05 pm